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KMID : 0377519900150020197
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Chung-Ang Journal of Medicine
1990 Volume.15 No. 2 p.197 ~ p.208
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Serologic Cross Reactions between Saline Extract of Trichinella Spiralis Muscle Larvac and Human Sera Infected with Trematodes
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Hong, Kyung-Pyo/홍경표
Kong, Yoon/Kang, Shin-Yong/Cho, Seung/공윤/강신영/조승열
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Abstract
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In Korea, authentic human or animal trichinellosis has never been reported until now. However, positive reaction to anti-Trichnella IgG antibody has not: uncommonly found in routine serologic screening for parasitic diseases when the crude saline ex-tract of T. spiralis is used as antigen in ELISA. In order to elucidate the nature of the positive antibody test for trichinellosis, human sera of trematodiasis, cestodiasis and normal control sera were tested against fortheir anti-Trichinella antibody. In normal cun, trol sera, no positive reactions were foun In cestodiasis such as cysticercosis; sparganosis and Taenia saginata infections, Z, j, of 49 examined samples were showed boderline positive reactions. In trematodia-" sis, however, the false positive reactions were more frequently encountered. Twentytwo or it 65 paragonindasis (33.8 561 1 of 15 donor. chiasis (6.6%), 2 of 2 metagonimiasis and y 1 1 3 of 4 fascioliasis were reacting positively. Sera from Trichinella infected rats showed significantly higher antibody levels to Paragonimus and Clonorchis antigens than thos,-´-~ to cystic fluid of Taenia solium metacestodes or sparganum antigens. When proteins in the crude saline extract of T. spiralis were separated by SDS-PAGE, a total of 25 bands were recognized. Of them, 3 protein bands of 45, 35 and 25 kDa were cross-reacted with the sera from trematodes infections. The Q three bands also showed strong reactions Lo Trichinella infected rats sera. These results by dicated that the false positive reaction for. anti- Trichinella IgG antibody should be taken into account when the crude saline extract : of T. sphwhs is used as a seroepiderniologi6l screening for trichinellosis. These false positive reaction was considered to be cau, ed by common antigenic proteins present in the crude saline extracts of both T.
muscle larvae and trematodes.
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